Table 1. Summary of intracellular pH (pH_i) measurements in single intercalated cells in mouse OMCD fragments.

	Initial pHi	Maximal aciditication, pHi	Na+ independent pHi recovery, ΔpH/min	Final pHi	No. of cells (OMCD fragments)
Control	7.20 ± 0.01	6.62 ± 0.01	0.040 ± 0.002	7.13 ± 0.01	133 (12)
Concanamycin	7.22 ± 0.01	6.63 ± 0.01	0.008 ± 0.001*	7.07 ± 0.01	179 (10)
Aldosterone, 1 nM	7.21 ± 0.01	6.61 ± 0.01	0.045 ± 0.001	7.07 ± 0.01	32 (3)
Aldosterone, 10 nM	7.20 ± 0.01	6.61 ± 0.01	0.093 ± 0.004*	7.07 ± 0.01	262 (20)
Aldosterone + concanamycin	7.19 ± 0.01	6.61 ± 0.01	0.019 ± 0.001*,**	7.09 ± 0.01	223 (12)
Spironolactone	7.22 ± 0.02	6.61 ± 0.01	0.038 ± 0.004	6.99 ± 0.02	31 (3)
Aldosterone + spironolactone	7.21 ± 0.01	6.62 ± 0.01	0.091 ± 0.004*	7.19 ± 0.01	132 (9)
Actinomycin	7.18 ± 0.01	6.62 ± 0.01	0.043 ± 0.003	7.00 ± 0.02	49 (3)
Aldosterone + actinomycin	7.26 ± 0.01	6.60 ± 0.01	0.071 ± 0.003*	7.07 ± 0.01	102 (8)
Cycloheximide	7.23 ± 0.01	6.60 ± 0.01	0.033 ± 0.003	6.95 ± 0.01	56 (5)
Aldosterone + cycloheximide	7.22 ± 0.01	6.60 ± 0.01	0.080 ± 0.002*	7.13 ± 0.04	169 (9)
Colchicine	7.20 ± 0.01	6.62 ± 0.01	0.037 ± 0.002	7.10 ± 0.02	55 (4)
Aldosterone + colchicine	7.22 ± 0.01	6.62 ± 0.04	0.035 ± 0.001	7.15 ± 0.01	146 (8)
Aldosterone + chelerythrine	7.28 ± 0.01	6.52 ± 0.02	0.030 ± 0.003***	7.21 ± 0.02	29 (3)
Chelerythrine	7.27 ± 0.02	6.47 ± 0.04	0.027 ± 0.003	7.22 ± 0.03	15 (3)

The statistical mean ± SEM are given. If not stated otherwise substances were used at the following concentrations: aldosterone, 10 nM; concanamycin, 200 nM; spironolactone, 10 μM; actinomycin, 10 μM; cycloheximide, 40 μg/ml; and colchicine, 10 μM. *, results significantly different from control; **, significantly different from concanamycin alone; ***, significantly different from 10 nM aldosterone (P < 0.001).