Table 2. Subcellular localization of the a4 vacuolar H+-ATPase subunit in single intercalated cells in the OMCD.
IC, % | PC, % | Apical, % | Subapical, % | Diffuse, % | Cells, n | |
---|---|---|---|---|---|---|
Control | 36.5 ± 2.1 | 63.5 ± 2.1 | 32.5 ± 2.2 | 56.8 ± 1.7 | 10.6 ± 1.9 | 265 |
Aldosterone | 35.5 ± 1.5 | 64.5 ± 1.5 | 77.2 ± 5.4* | 17.6 ± 4.1* | 5.2 ± 2.0 | 297 |
PC, principal cells; IC, intercalated cells. *, Significantly different from control (P < 0.001). Intercalated cells were classified according to the predominant intracellular staining of the a4 subunit. Aldosterone injection resulted in a percentual increase in intercalated cells with a predominantly apical staining.