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Reduction of proteins of pea leaf mitochondria by Trx after treatment with N-ethylmaleimide to remove background SH groups. The mutant affinity column eluate was either not treated (A) or reduced by Trx (B) using E. coli Trx, NADPH, and NTR. After reduction, the proteins were derivatized with monobromobimane and subjected to 2D electrophoresis. (C) As in B, but stained for protein.
