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. Author manuscript; available in PMC: 2013 Feb 12.
Published in final edited form as: Environ Mol Mutagen. 2012 Mar 29;53(5):334–342. doi: 10.1002/em.21692

Fig. 1.

Fig. 1

Avy and CabpIAP loci (A) The Avy allele contains a contra-oriented IAP insertion within pseudoexon 1A (PS1A) of the Agouti gene. A cryptic promoter (short arrowhead labeled Avy ectopic) drives constitutive ectopic Agouti expression. Transcription of the Agouti gene normally initiates from a developmentally regulated hair-cycle specific promoter in exon 2 (short arrowhead labeled A, a wildtype). The location of the bisulfite-converted genomic reverse primer for amplifying the Avy IAP is underlined. (B) The CabpIAP metastable epiallele contains a contra-oriented IAP insertion within intron 6 of the murine CDK5 activator-binding protein (Cabp) gene, resulting in short aberrant transcripts originating from the 5′-LTR of the IAP (short arrowhead labeled Cabp AT1a,b). Aberrant transcripts also originate at the normal transcription start site (short arrowhead labeled Cabp wildtype) and truncate 5′ of the IAP insertion (Cabp AT2 and AT3). Normal Cabp transcription covers 14 exons, resulting in a 2-kb transcript. The location of the bisulfite-converted genomic reverse primer for amplifying the CabpIAP locus is underlined.