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. 2012 Dec 21;5(3):789–792. doi: 10.3892/etm.2012.871

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Copyright © 2013, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Figure 3. — Effects of MECO and MECB on Sp1 expression. (A) HSC-2 cells were treated with DMSO or various concentrations (300, 600 and 900 μg/ml) of MECO or MECB for 48 h. The effects of MECO and MECB on Sp1 expression were analyzed by western blot analysis. (B) HSC-2 cells were transfected with siRNA (siCon or siSp1) for 48 h. The cell viability was estimated by trypan blue exclusion assay and whole-cell lysates were analyzed by western blot analysis using antibodies against Sp1, PARP and actin. MECO, methanol extracts of C. officinale Makino; MECB, methanol extracts of C. bursa-pastoris; Sp1, specificity protein 1; PARP, poly (ADP-ribose) polymerase.