Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 Feb 11;210(2):287–300. doi: 10.1084/jem.20122149

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Aichinger et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 2. — Direct presentation of mitochondrial or membrane-bound PCC by TECs is sufficient for negative selection, but differs in its requirement for autophagy. (a) Subset composition of antigen non-tg (no Ag), ePCC, or mPCC thymi 4–6 wk after transplantation into AND × Ciita^−/− recipients. The mean frequency ± SD of CD4SP cells (top row) and of AND-TCR⁺ cells (Vα11⁺Vβ3⁺) among gated CD4SP thymocytes (bottom row) is indicated. Data are representative of ≥11 chimeras per group. (b) Subset composition of WT × Atg5^−/−, ePCC × Atg5^−/−, or mPCC × Atg5^−/− thymi 4–6 wk after transplantation into AND × Ciita^−/− recipients. The mean frequency ± SD of CD4SP cells (top row) and of AND⁺ cells (Vα11⁺Vβ3⁺) among gated CD4SP thymocytes (bottom row) is indicated. Data are representative of ≥10 chimeras per group. (c) Frequencies of CD4SP Vα11⁺Vβ3⁺ cells among total thymocytes in a and b. (d) Relative abundance of the respective tg mRNA in purified total TECs from ePCC and mPCC tg thymi on Atg5^+/− or Atg5^−/− background as determined by qPCR. TECs were isolated from grafted E14-16 thymi 4–5 wk after transplantation into WT recipients. Values (arbitrary units; AU) are normalized to expression in TECs from ePCC Atg5^−/− thymi and indicate the mean ± SD from three independent biological replicates.