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. 2013 Feb 11;210(2):287–300. doi: 10.1084/jem.20122149

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© 2013 Aichinger et al.

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Figure 3. — GCL is targeted to autophagosomes, whereas a difference by 1 aa in GCL_G120A abolishes autophagosomal routing. (a) Schematic representation of the GCL and GCL_G120A fusion proteins. (b) During autophagy, LC3 is covalently coupled to the inner and outer autophagosomal membrane. After fusion with lysosomal compartments, LC3 in the autophagosomal lumen is broken down proteolytically. (c) HEK 293T cells were transfected with expression vectors encoding for GCL or GCL_G120A. Subsequently, cells were either cultured in full medium or transferred to starvation medium. Bars, 20 µm. Images are representative of two independent experiments.