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. 2013 Feb 11;210(2):287–300. doi: 10.1084/jem.20122149

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© 2013 Aichinger et al.

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Figure 4. — GCL and GCL_G120A are equally expressed in mTECs of tg mice, yet differ in their direct presentation by mTECs. (a) Diagram of the Aire-GCL and Aire-GCL_G120A BAC transgenes driving expression of the fusion proteins in mTECs. (b) Thymic stromal cells from WT (gray), Aire-GCL (blue), and Aire-GCL_G120A (red) mice were analyzed by flow cytometry for GFP-fluorescence. Data are representative of two independent experiments, each with cells from three or more animals per genotype. (c) Abundance of tg mRNAs in thymic stromal cells from Aire-GCL or Aire-GCL_G120A mice as determined by qPCR. Values are normalized to mTEC^hi from Aire-GCL mice and indicate the mean ± SD from three biological replicates. (d) NFAT-GFP reporter hybridoma cells expressing the hCRP_89-101 specific DEP-TCR were co-cultured with mTECs (filled bars) or DCs (open bars) from Aire-GCL or Aire-GCL_G120A mice. The mean frequency ± SD of GFP⁺ hybridoma cells is indicated. Data summarize three independent experiments with material from six or more thymi.