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. 2013 Feb 11;210(2):321–337. doi: 10.1084/jem.20121527

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© 2013 Kannan et al.

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Figure 2. — Notch pathway expression in human AML lines. (A) mRNA expression of Notch1–4 by QRT-PCR. Asterisk denotes lack of Notch2 mRNA in K562 line. (B) mRNA expression of Notch target gene HES1 in T-ALL and AML lines by QRT-PCR. Log scale was used, and triplicate samples were analyzed. (C) Immunoblot of AML and T-ALL lines probed for Notch1, Notch2, cleaved Notch1/2, and β-actin. Asterisk denotes K562 line with undetectable Notch2 protein expression. Representative of three blots. (D) Immunoblot of AML and T-ALL lines probed for Notch ligands Jagged1 and DLL1, as well as cleaved Notch1/2 and β-actin. Representative of two blots. (E) Flow cytometry data for Notch1 and Notch2 cell surface expression on AML and T-ALL lines, normalized to isotype controls in each cell line (log scale; mean ± SD). Triplicate samples analyzed.