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. 2013 Feb 11;210(2):389–399. doi: 10.1084/jem.20121970

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© 2013 Muellenbeck et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 1. — Memory B cell sorting. (A) Relative 3D7^Luc neutralizing activity of 100 µg/ml purified serum IgG from study participants in comparison to polyclonal serum IgG preparations from nonimmune controls (0% neutralization) and 50 mM chloroquine (100% neutralization, dashed gray line). Dots represent individual samples. Selected donors for antibody cloning are indicated. (B) Gating strategy for the flow cytometric isolation of CM and AtM. Representative plots from MP070 are shown. (C) Frequency of circulating CM and AtM. (D) IgG GMZ2 reactivity in serum as measured by ELISA. The dotted line shows a Pf-naive control serum. (E) Flow cytometric analysis of GMZ2-reactive CM and AtM in peripheral blood from MP036, MP070, and MP071 compared with a malaria naive donor. (F) Frequency of circulating GMZ2-reactive CM and AtM in all donors as determined in E. Red bars indicate arithmetic mean.