Table 1.
Iron content of L. amazonensis cultured in iron-poor medium
| Days of culture in iron-depleted medium | WT | Δlit1 |
| nmol/mg protein | nmol/mg protein | |
| 0 | 8.8 ± 0.7 | 8.7 ± 0.4 |
| 1 | 6.7 ± 0.6 (P = 0.0424)a | 5.5 ± 0.9 (P = 0.0158)a |
| 2 | 9.3 ± 1.9 (P = 0.1978) | 5.8 ± 1.2 (P = 0.0798) |
| 3 | 10.88 ± 1.7 (P = 0.0369)a | 5.9 ± 1.1 (P = 0.0451)a |
Determination of total cellular iron content of L. amazonensis WT and Δlit1 parasites was performed using ferrozine-based quantitation (Riemer et al., 2004). The data represent the mean and SD of five independent experiments.
a
Indicates significant differences (P ≤ 0.05) from day 0 values (two-tailed Student’s t test).