Calpain inhibition decreased apoptosis in optic nerve in EAE Rats. Representative (A) Western blots and corresponding (B) Bax:Bcl-2 ratio; (C) bar diagrams (densitometry) showing levels of expression of 24 and 21 kD Bax, 26 kD Bcl-2, 18 kD caspase-8, 15 kD tBid, 120 kD SBDP, 12 kD caspase-3, and 87 kD PARP-1 in optic nerve of EAE rats (n ≥ 3 per group, *p ≤ 0.05 compared with untreated control (Con); #p ≤ 0.05 compared with EAE-CP0). (D) Agarose gel electrophoresis of genomic DNA samples for detection of internucleosomal DNA fragmentation.