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. 2004 Feb 6;101(7):1828–1833. doi: 10.1073/pnas.0306417101

Fig. 4.

Fig. 4.

Equilibrium sedimentation analytical ultracentrifugation of WSK-3 and AgTx2-DNP. (a) 17 μM WSK-3 monitored at 280 nm (o). Data were fit to a monomer–tetramer–12-mer equilibrium by using a macro in igor-pro (WaveMetrics, Lake Oswego, OR). (b) AgTx2-DNP (17 μM) in the presence (red circle) and absence (green circle) of 17 μM WSK-3 tetramer, monitored at 360 nm to detect only the AgTx2-DNP. (c) Control with BSA (36 μM) plus AgTx2-DNP (17 μM) monitored at 280 nm (blue square) to monitor the BSA, and at 360 nm (green square) to monitor AgTx2-DNP.