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| Experiment | [Tox] bound, μM | Stoichiometry |
|---|---|---|
| WSK-3mon (40 μM) + Tox (10 μM) | 9.0 | [WSK-3]/[Tox]bound = 4.4 |
| WSK-3mon (40 μM) + Tox (20 μM) | 9.7 | [WSK-3]/[Tox]bound = 4.1 |
| BSA (20 μM) + Tox (44 μM) | 0 | |
| CA (47 μM) + Tox (23 μM) | 0 |
Initially, WSK-3 and AgTx2-DNP (Tox) are on side a, whereas buffer is on side b. At equilibrium, the concentration of bound toxin is given by [Tox]bound = [Tox]a - [Toxfree]a = [Tox]a - [Tox]b.
