PGE2-G increases the levels of inositol 1,4,5-trisphosphate (IP3). IP3 levels in lysates of cells treated with 50 nM PGE2-G, 50 nM PGE2, 50 nM PGF2α, or 1 μM thapsigargin were measured. Results from a typical experiment are shown. Error bars represent standard deviation in triplicate samples of a single experiment.