Fig. 5.

PGE₂-G induces the activity of PKC in RAW264.7 membrane fractions. RAW264.7 cells were treated for 5 min with vehicle, 0.1 μM ionomycin/0.1 μg/ml PMA, 1, 10, or 50 nM PGE₂-G, 50 nM PGF₂α, 50 nM PGE₂, or 1 μM thapsigargin. Cells were lysed, and PKC activity in membrane fractions was measured as described in Experimental Procedures. The experiment was performed at least five times in duplicate. Results shown are from a typical experiment. Error bars represent the range in duplicate samples of a single experiment.