Table 1. Binding of PGE2 and PGE2-G to prostanoid receptors.
EP1 | EP2 | EP3 | EP4 | DP | TP | FP | IP | |
---|---|---|---|---|---|---|---|---|
PGE2-G | 979 | >19,800 | 378 | 737 | 13,300 | >22,300 | 16,700 | >20,600 |
PGE2 | 10.1 | 0.82 | 0.7 | 0.7 | (307) | (29,000) | (119) | (>100,000) |
Membrane fractions from HEK 293 cells stably overexpressing EP1-4, DP, FP, TP, and IP receptors were analyzed for 3H-PGE2 binding in the presence or absence of cold PGE2-G. The values in parentheses are for comparison and are taken from an identical assay performed by Abramovitz et al. (6).