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. 2012 Dec 10;5(2):561–566. doi: 10.3892/etm.2012.855

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Copyright © 2013, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Figure 2. — Apoptotic rates of HeLa cells following incubation with BAK and NK cells. HeLa cells without treatment were used as a blank control. Early and late apoptotic cells were combined to calculate the percentage of cell apoptosis. (A) BAK cells had a significant impact on apoptosis in HeLa cells with 24.2±9.2% apoptotic cells compared with the NK control (13.45±5.1%) and the blank control (1.25±0.8%). The NK group also showed significant apoptosis compared with the blank control (^*P<0.05). (B) Cell apoptosis was evaluated by using FITC Annexin V/propidium iodide-double staining, and the stained HeLa cells were analyzed by fluorescent-activated cell sorting (FACS). The figure shown is representative of a set of experiments. BAK, BCG-activated killer; NK, natural killer; PI, propidium iodide; BCG, Bacillus Calmette-Guerin.