Skip to main content
. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: J Mol Cell Cardiol. 2012 Mar 23;52(6):1249–1256. doi: 10.1016/j.yjmcc.2012.03.008

Fig. 5.

Fig. 5

MSC derived paracrine factors induce pro-survival Akt signaling in VMs. (A) Western blotting shows 3 representative experiments of cell lysate from VMs that were treated with Ctrl or ConT (15 min). Immunoblotting for p-Akt(Ser473), p-GSK-3α/β(21/9), and pan-Akt as a loading control are shown. (B) The percentage of VMs surviving after isolation is significantly increased in CoC (gray) and ConC (white) and ConT (C) conditions in comparison to Ctrl (black). (D) Western blot of Akt phosphorylation for HL-1 cells treated with ConT (30 min) derived from mMSCs, HL-1 cells, mouse fibroblasts or hMSCs show that increased Akt phosphorylation is mediated primarily by MSCs. Total Akt (pan-AKT) and GAPDH are shown as loading controls.

HHS Vulnerability Disclosure