Fig. 7.
Lack of PLN does not prevent ConT induced shortening of Ca transient. (A) Ca transient amplitude and (B) τCa in PLN−/− VMs recorded during superfusion with Ctrl (O) or ConT (■). (C) Western blotting analysis of 3 representative experiments shows that while ConT (15 min) activates Akt phosphorylation (p-AktSer473) PLN phosphorylation (p-PLNThr17) in WT myocytes remains almost unchanged. Total PLN and GAPDH are shown as loading controls.