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Long-wavelength modulation of AcGFP. Modulation frequency dependence of enhancement for immobilized (black) and diffusing (red) AcGFP molecules. The frequencydependent modulation depth was computed by Fourier transforming each time trace, normalizing by the number of data points, and dividing each by its DC component. Inset: Fourier transform of modulated fluorescence of AcGFP at 1 Hz. Square wave modulation also produces odd harmonics.
