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. 2013 Jan 7;5(3):701–706. doi: 10.3892/etm.2013.887

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Copyright © 2013, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Figure 2. — Isolation and culture of human adipose-derived stem cells (hASCs) in vitro [(A) P0 (B) P1 (C) P2 (D) P3, ×100 magnification]. (Ea-d) Flow cytometry performed on the hASCs in the 3rd passage showed that isolated hASCs positively expressed CD13, CD44, CD90, CD105 and negatively expressed CD14, CD34. (F) Adipogenic differentiated ASCs were positively stained by Oil Red O. (G) Osteogenic differentiated adipose-derived stem cells (ASCs) were positively stained by Alizarin red. (H) Protein microarray analysis of ASC-CM. ASC-CM, ASC-conditioned medium. P0, primary ASCs; P1, passage 1; P2, passage 2; P3, passage 3.