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. Author manuscript; available in PMC: 2014 Jan 7.
Published in final edited form as: Mol Pharm. 2012 Dec 17;10(1):360–377. doi: 10.1021/mp300496x

Table 2.

Molecular characteristics of SODI-POx conjugates.

Conjugatesa Linker Modification degreeb Residual activity (% unmodified)c Tm (°C)d,e
SOD1-(cc)-P(MeOx-b-BuOx) DSS 2.2 36 88.65
SOD1-(ss)-P(MeOx-b-BuOx) DSP 3.5 47 N/A
SOD1-(cc)-P(EtOx-b-BuOx) DSS 2.5 34 88.38
SOD1-(ss)-P(EtOx-b-BuOx) DSP 2.3 31 N/A
a

Here “-(cc)-” and “-(ss)-” designate SOD1-POx conjugates containing stable and degradable bonds formed by DSS and DSP, respectively.

b

Counting per SOD1 dimer as determined by TNBS assay.

c

Determined by pyrogallol auto-oxidation assay.

d

Determined by DSC.

e

Tm of unmodified SOD1 is 92.14 °C.