Fig. 3.
RNAi-mediated inducible gene suppression is reversible. (A) Western blot analysis showing 93% fold reduction in p53 protein levels in cells induced with 5 μM murA (+murA) relative to uninduced (–murA) at 72 h. After murA removal, the fold recovery in p53 levels is 20% (48 h) and >90% (96 h). (B) Phase-contrast microscopy showing morphology of U87MG cells either uninduced (–murA) or induced with 5 μM murA (+murA) for 72 h and at 48 and 96 h after murA removal.