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. 2004 Feb 4;101(7):1927–1932. doi: 10.1073/pnas.0306111101

Fig. 4.

Fig. 4.

Inducible suppression of MyoD gene expression in a murine endothelial cells. (A) Cells stably and inducibly expressing MyoD shRNA (MyoD-SP) and nonspecific shRNA (NON-SP) were treated with 5 μM murA. Whole-cell extracts were prepared at 72 h after induction and analyzed by Western blot for MyoD and β-tubulin. Fold reduction of protein level is >95% for MyoD (MyoD-SP) relative to nonspecific shRNA. (B) Stable cells carrying MyoD specific shRNA (MyoD-SP) and nonspecific shRNA (NON-SP) were induced with 0.0 (lane 1), 0.5 (lane 2), 1.0 (lane 3), 3.0 (lane 4), and 5.0 (lane 5) μM murA and subjected to Western blot for MyoD and β-tubulin. Fold reduction of MyoD protein level for shRNA (MyoD-SP) is 5% (lane 2), 25% (lane 3), 72% (lane 4), and >95% (lane 5) relative to uninduced sample (lane 1). Fold reduction in MyoD was normalized against β-tubulin for each band. shRNA used for the suppression of MyoD gene expression was identified in a high-throughput screen (19).