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. 2004 Feb 9;101(7):1933–1938. doi: 10.1073/pnas.0305476101

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Fig. 3. — Flexible use of primer ratios in LATE-PCR. LATE-PCR primers at 1:10 (A), 1:40 (B), and 1:100 (C) ratios wherein (T_m^L – T_m^X) ≥ 0 have similar amplification efficiencies. In contrast, conventional symmetric PCR primers at a 1:100 ratio (D), wherein (T_m^L – T_m^X) < 0 are inefficient. Primer ratios were created by holding the concentration of the excess primer constant and then decreasing the concentration of the limiting primer. , Asymmetric reactions; ▪, symmetric reactions.

Inline graphic — Flexible use of primer ratios in LATE-PCR. LATE-PCR primers at 1:10 (A), 1:40 (B), and 1:100 (C) ratios wherein (T_m^L – T_m^X) ≥ 0 have similar amplification efficiencies. In contrast, conventional symmetric PCR primers at a 1:100 ratio (D), wherein (T_m^L – T_m^X) < 0 are inefficient. Primer ratios were created by holding the concentration of the excess primer constant and then decreasing the concentration of the limiting primer. , Asymmetric reactions; ▪, symmetric reactions.