Fig. 5.
Low-Tm molecular beacons improve allele discrimination over a wider range of detection temperatures compared with high-Tm probes. Melting curves of high-Tm (A) and low-Tm (B) molecular beacons specific for the wild-type TSD 1421 HEXA allele bound to various targets illustrate the increased allele-discrimination capacity of low-Tm molecular beacons. -×-×-, No target; ———, correct target; ······, 1-nt mismatched target. Arrows indicate the temperature discrimination window between matched and mismatched targets.