Table 1. TSD 1278 HEXA primers.

Method	Primer	Sequence	Concentration, nM	Tm*, °C	ΔTm
Symmetric PCR	TSD1	5′-CCTTCTCTCTGCCCCCTGGT-3′	1,000	64.8	0
	TSD2	5′-GCCAGGGGTTCCACTACGTAGA-3′	1,000	64.3
Conventional asymmetric PCR	TSD1	5′-CCTTCTCTCTGCCCCCTGGT-3′	25	58.9	-5
	TSD2	5′-GCCAGGGGTTCCACTACGTAGA-3′	1,000	64.3
LATE-PCR†	Modified TD1	5′-GCCCTTCTCTCTGCCCCCTGGT-3′	25	64.0	0
	TSD2	5′-GCCAGGGGTTCCACTACGTAGA-3′	1,000	64.3

^*Concentration-adjusted T_m calculated according to the nearest-neighbor formula.

^†Nucleotides underlined and in bold were added to match T_m values.