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. 2004 Feb 9;101(7):1951–1956. doi: 10.1073/pnas.0307145101

Fig. 3.

Fig. 3.

Analysis of secreted particles. (A) The kinetics of the release of protein E from BHK-21 cells transfected with wild-type or mutant RNAs was monitored by ELISA. (B) Particles secreted by mutant C(▵28–89)-S were analyzed on a discontinuous (10%, 35%, and 50% as indicated below) sucrose gradient and compared with virus and RSP controls. (C) The buoyant density of particles secreted by mutant C(▵28–89)-S was determined by equilibrium sucrose gradient centrifugation in comparison with RSPs. (D) SDS/PAGE of viral and subviral particles. Particles secreted from cells transfected with C(▵28–89)-S were fractionated and compared with RSP and wild-type virus preparations. The positions of the structural proteins E, M (and its precursor, prM), and C are indicated.