Fig. 4.
CPH inhibit production of TH1 and TH2 cytokines. PBT were incubated with peptides M/W (black bars) or without peptides (white and gray bars) and then stimulated via CD2 (gray and black bars) for 48 h (A) or 4 h (B) or left unstimulated (white bars). (A) Secretion of IFN-γ and IL-10 into culture supernatants. Data are means ± SE of four (IFN-γ) and three (IL-10) independent experiments with cytokine amounts produced by stimulated cells in the absence of CPH taken as 1. (B) mRNA expression (quantitative RT-PCR) of IFN-γ and IL-10. Normalized mRNA levels of stimulated cells without CPH were taken as 1 (means ± SE of four blood donors). Effects were similar after 2 h of CD2 stimulation (not shown).