Fig. 5.

CPH inhibit CD2 receptor capping. PBT were preincubated without (Top and Middle) or with (Bottom) peptides M/W. Caps were induced with CD2 mAb and anti-mouse-Cy3 mAb (CD2 crosslinking) or cells were only incubated with CD2 mAb (no crosslinking). After fixation and permeabilization, cells were counterstained for cofilin (green, Left). For samples without crosslinking, anti-mouse Cy3-mAb was included to visualize CD2 distribution (red, Center). Colocalization of red and green fluorescence appears yellow (Right).