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. 2004 Feb 5;101(7):1963–1968. doi: 10.1073/pnas.0308490100

Fig. 4.

Fig. 4.

Effect of multimerization of CR on CRL binding. (A) CR-Fcmut (○) was able to bind to bLH immobilized on 96-well enzyme immunoassay and RIA plates. Cross-linking of the recombinant protein with a mouse mAb F(ab)2 fragment anti-human Fc (•) greatly enhanced the binding of CR-Fcmut to bLH. This activity was abrogated by the W117A (□) amino acid substitution, and cross-linking of CRW117A-Fcmut (▪) could not restore any detectable binding. (B) The effect of various amounts of cross-linking mouse F(ab)2 anti-human Fc Ab on the ability of CR-Fcmut to bind to bLH. Gel filtration analysis confirmed the formation of complexes of CR-Fcmut and mouse mAb F(ab)2 fragment anti-human Fc.