Table 1.
Characteristics of OXA-181-producing Klebsiella pneumoniae clinical isolates
| Isolate | Hospital | Specimen | Date of Isolation | ST |
16S rRNA methylase gene |
β-lactamases |
Plasmids |
MIC (mg/L) |
|
|
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Size (kb) | Replicon(s) | IMP | MEM | ETP | FOX | CTX | CAZ | AZT | COL | TGC | LEV | GEN | AMK | |||||||
| KPO8 |
Hospital A |
Tracheal aspirate |
18/01/2011 |
17 |
armA |
TEM-116, SHV-11, CTX-M-15, OXA-1 |
~97, ~170 |
A/C |
>32 |
>32 |
>32 |
>256 |
>256 |
64 |
>256 |
0.125 |
2 |
0.25 |
>256 |
>256 |
| KPO9 |
Hospital A |
Sputum |
8/02/2011 |
17 |
armA |
TEM-1, SHV-11, CTX-M-15, OXA-1 |
~9, ~150, ~170 |
A/C |
>32 |
>32 |
>32 |
>256 |
>256 |
64 |
>256 |
16 |
>256 |
>256 |
>256 |
>256 |
| KPO7 |
Hospital A |
Tracheal aspirate |
9/12/2010 |
14 |
armA |
TEM-1, SHV-1, CTX-M-15, OXA-1, OXA-9 |
~1.5, ~50, ~170, ~194 |
A/C, F |
>32 |
>32 |
>32 |
>256 |
>256 |
>256 |
>256 |
0.25 |
12 |
>256 |
>256 |
>256 |
| KPO26 |
Hospital A |
Sputum |
14/05/2011 |
14 |
ND |
TEM-1, SHV-1, CTX-M-15, OXA-1, OXA-9 |
~1.6, ~194 |
A/C |
>32 |
>32 |
>32 |
>256 |
>256 |
>256 |
>256 |
32 |
4 |
>256 |
>256 |
96 |
| KPO6916 |
Hospital A |
Blood |
2/03/2012 |
14 |
armA |
TEM-1, SHV-1, CTX-M-15, OXA-1, OXA-9 |
~1.6, ~50, ~170, ~194 |
A/C |
8 |
8 |
>32 |
>256 |
>256 |
>256 |
>256 |
0.25 |
16 |
>256 |
>256 |
>256 |
| KPO7431 |
Hospital B |
Urine |
22/02/2012 |
14 |
armA |
TEM-1, SHV-11, CTX-M-15, OXA-1, OXA-9 |
~1.5, ~50, ~170, ~194 |
A/C, F |
4 |
4 |
>32 |
>256 |
>256 |
>256 |
>256 |
0.25 |
8 |
>256 |
>256 |
>256 |
| KPO83 |
Hospital C |
Urine |
30/12/2011 |
14 |
armA |
TEM-1, SHV-1, CTX-M-15, OXA-1, OXA-9 |
~170 |
A/C |
32 |
8 |
>32 |
>256 |
>256 |
>256 |
>256 |
0.5 |
16 |
32 |
>256 |
>256 |
| KPO9–T |
― |
― |
― |
― |
armA |
OXA-181, CTX-M-15 |
~170 |
A/C |
2 |
0.5 |
0.5 |
2 |
3 |
1 |
0.75 |
0.125 |
0.125 |
0.002 |
>256 |
>256 |
| KPO26 –T |
― |
― |
― |
― |
ND |
OXA-181, OXA-9 |
~194 |
A/C |
2 |
0.19 |
0.25 |
12 |
6 |
2 |
6 |
0.125 |
0.125 |
0.003 |
0.125 |
1 |
| E. coli J53 | ― | ― | ― | ― | ― | ― | ― | ― | 0.125 | 0.016 | 0.04 | 1 | 0.064 | 0.25 | 0.064 | 0.125 | 0.064 | 0.004 | 0.125 | 0.125 |
Legend:
MIC, minimum inhibitory concentration; IPM, imipenem; MEM, meropenem; ETP, ertapenem; FOX, cefoxitin; CTX, cefotaxime; CAZ, ceftazidime; AZT, aztreonam; COL, colistin; TCG, tigecycline; LEV, levofloxacin; GEN, gentamicin; AMK, amikacin; KPO8, KPO9, KPO7, KPO26, KPO6916, KPO7431, KPO83 carbapenem-resistant K. pneumoniae clinical isolate; KP09-T, KP026-T, K. pneumoniae transconjugant from KP09, KP026; J53, E. coli recipient strain.