Fig. 3.
pmel-1 splenocytes differentially polarize either to a TEM-orTCM-like population when cultured in IL-2 or IL-15, respectively. Splenocytes from a pmel-1 Tg mouse were primed with 1 μM hgp10025–33 peptide and cultured in culture media containing 10 ng/ml rhIL-2 or 10 ng/ml rhIL-15. On d 7, FACS analysis for surface expression of the activation markers CD25, CD44, and CD69 and the lymph node homing markers CD62L and CCR7 was performed. Naïve pmel-1 cells were obtained from splenocytes of an age-matched littermate. Results from a representative experiment are shown after gating on propidium iodide-negative and CD8+ T cells.