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. 2013 Jan 16;14:23. doi: 10.1186/1471-2164-14-23

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Copyright ©2013 Affara et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(a) Quantification of active caspase-3 and −7 in three independent pools of 10 HUVEC isolates treated with either a non-targeting siRNA for 48hrs or siRNA against VASH1 for 48 hrs. HUVECs were treated 24hrs post transfection with survival factor deprived (SFD) conditions for 24hrs before measurement. A significant difference was observed between the VASH1 knockdown and the siRNA control in the SFD condition (P = 0.0009, paired two tailed t-test). (b) Quantification of the ADP:ATP ratio in three pools of 10 HUVEC isolates treated with either a non-targeting siRNA for 48hrs or siRNA against VASH1 for 48hrs. HUVECs were treated 24hrs post transfection with SFD conditions for 24hrs before measurement (P = 0.02). P = Pool.