Table 1.

Kinetic parameters for the binding of PhaR to DNAs and P(3HB) on the 27-MHz QCM^a

Targetsb	DNA sequencec	kond(10-4 M-1 s-1)	koffc(10-3 s-1)	Kde(10-7 M)
Control DNAf	5′bio-TCGTTTAACGAGCCCGTATTTTCCCCTCTACCTTTTAGAGGACACCTAAC-3′	0.4	0.7	18
phaP1 promoterg	5′bio-GGCGCATTTCTTATTTGGTGCGCCGCAACAATTCCTATTTTAGGGGCGCC-3′	6.0 ± 0.4	1.7 ± 0.4	3.2 ± 0.9
phaR promoterf	5′bio-TCACGCGTTTAGCCATAGCGGGCGCGGTAGACGAACAACAGCACGGCCGG-3′	0.5	0.9	18
am-P(3HB)g		7.0 ± 3.8	-h	-h

^a 10 mM HEPES buffer solution (pH 7.4) containing 150 mM NaCl and 0.002% Tween 20, 25°C. ^b 5′-biotinylated dsDNA immobilized on an avidin-covered QCM plate.

Amorphous-P(3HB) thin film was prepared by casting chloroform solution (1.0 wt%) of the isotactic P[(R), (S)-3-hydroxybutyrate]. ^c The bold type indicates the DNA binding site of PhaR revealed by DNase I footprinting (4). ^dk_on and k_off were obtained from Eq. 4. ^eK_d was calculated as k_off/k_on. ^f The data are from 2 independent experiments. ^g Amorphous P(3HB); the data are from 3 independent experiments. ^hK_d value could not be calculated due to a negative k_off value.