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. 2013 Feb 12;8(2):e56234. doi: 10.1371/journal.pone.0056234

Figure 5. BA-mediated protein degradation is dependent on the UPS.

Figure 5

(A) Trypan blue exclusion assay showed that 1 µM MG132 antagonized cell death in BA treated LNCaP cells (24 h; *, P<0.02). Western blot analysis showed that MG132 blocked the BA-mediated increase of cl-PARP and decrease of AR, AKT, and Mcl-1 proteins. (B) Western blot analysis showed that transfected cyclin D1 T286A mutant but not cyclin D1 wild type protein was resistant to BA-mediated degradation (24 h) in LNCaP cells. (C) Proteasome activity assay showed no significant effect in LNCaP cells treated with BA for 8, 24, and 48 h. Addition of MG132 (MG) to BA resulted in decreased activity.