Table1. Segregation analysis showing a pollen transmission defect associated with a double knockout of cngc7/8.

Cross	F1	Segregation of +/− T-DNA
Female X Male	Total	Expect%a	Observed%	p- valueb
Crosses with single mutants
cngc7-1+/−; SELFED	559	75	74.1f, e	0.99
cngc7-3+/−; SELFED	178	75	74.7c	0.99
cngc8-1+/−; SELFED	1347	75	74.5d	0.97
cngc8-2+/−; SELFED	1409	75	77.4d	0.6
WT X cngc7-1+/−	37	50	54c	0.95
WT X cngc7-3+/−	71	50	51c	0.99
WT X cngc8-2+/−	206	50	50d	1
Crosses with double mutants (one gene −/−, second gene +/−)
cngc7-1−/−, 8-2+/−; SELFED	637	75	50d	<0.0001
cngc7-1+/−, 8-2−/−;SELFED	599	75	47.7g,h	<0.0001
cngc7-3+/−, 8-1−/−; SELFED	76	75	51c,h	≤0.06
cngc7-1−/−, 8-2+/− X WT	143	50	55d	0.7
cngc7-3−/−, 8-1+/− X WT	308	50	55d	0.5
WT X cngc7-1+/−, 8-2−/−	727	50	0e	<0.0001
WT X cngc7-1−/−, 8-2+/−	756	50	0d	<0.0001
WT X cngc7-3−/−, 8-1+/−	5283	50	0d	<0.0001

^aExpected percentages based on Mendelian segregation.

^bSignificance determined by the Pearson’s Chi-Squared test with two degrees of freedom.

^cPCR genotyping.

^dMutant allele scored by Sulf ^r marker.

^eMutant allele scored by Basta^r maker.

^f117 by PCR genotyping.

^g313 by PCR genotyping.

^hno homozygous double knockout found.