Table 2. Consequences of T cell depletion for the establishment of challenge virus in naïve and primed Ig^-/- mice.

mAb depletion		Virus recovery from naïve and primed mice				Virus titers (log10 PFU/ml) and priming
CD4	CD8	Virus	Naïve	γHVCam	M3LacZ	Naïve	γHVCam	M3LacZ
-	-	Total	6/6	6/6	0/6	4.9 ± 0.2	2.3 ± 0.5	0
		GFP+	6/6	3/6	0/6	2.4 ± 0.2	1.12 ± 0.3	0
+	-	Total	6/6	5/6	5/6	3.9 ± 0.3	2.5 ± 0.5	1.6 ± 0.5
		GFP+	6/6	1/6	0/6	2.3 ± 0.3	1.1 ± 0.3	0
-	+	Total	6/6	6/6	3/6	4.6 ± 0.3	3.1 ± 0.2	1.1 ± 0.6
		GFP+	6/6	0/6	0/6	2.7 ± 0.4	0	0
+	+	Total	6/6	6/6	6/6	5.7 ± 0.2	5.1 ± 0.2	3.8 ± 0.1
		GFP+	6/6	6/6	6/6	3.5 ± 0.1	3.4 ± 0.3	1.8 ± 0.1

The μMT mice were primed i.p. with 1 × 10⁴ PFU of γHVCam or M3LacZ for 2 months. They were then dosed i.p. with the GK1.5 mAb to CD4 and/or the 2.43.1 mAb to CD8 three times before and every second day after i.n. challenge with 5 × 10⁶ PFU of 1:1 γHVW+BAC-GFP. Age-matched mice were used as naïve controls. Lungs were harvested on d5, homogenized, and assayed for PFU. The GFP⁺ plaques were counted by immunofluorescence before fixing and staining with Giemsa. The results are expressed as log₁₀ mean ± SE from six mice.