Table 3. Further establishment of latency after secondary challenge of M3LacZ-primed Ig^-/- mice.

	M3LacZ priming*	γHV68 challenge	T cell depletion	Lung titer, PFU†	Spleen latency
Line					Reciprocal LDA titer‡	Percent X-Gal+§¶
1	-	+	-	0	3.9 ± 0.1	0
2	-	+	+	3.1 ± 0.5	3.2 ± 0.8	0
3	+	-	-	0	4.7 ± 0.6¶	100
4	+	+	-	0	3.7 ± 0.6	23 ± 23
5	+	+	+	3.6 ± 0.6	2.8 ± 0.3	29 ± 15

^*The mice were infected i.p. with 1 × 10⁴ PFU of M3LacZ, then challenged i.n. 2 months later with 5 × 10⁶ PFU of 1:1 γHVW+BAC-GFP, together with naïve (-) controls. After another month, groups of naive and primed challenged mice were depleted for 10 days by the i.p. inoculation of mAbs to CD4 and CD8.

^†Mean ± SD log₁₀ per ml for groups of five mice. No “green” BAC-GFP or “blue” M3LacZ was detected.

^‡Log₁₀ reciprocal frequency of infected spleen cells detected by LDA. The BAC-GFP “green” virus was found in only three of five naïve, virus-challenged T cell-depleted mice.

^§Percentage of positive cells containing M3LacZ-derived “blue” plaques.

^¶Virus was detected in four of five mice.