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. 2013 Feb 12;8(2):e56309. doi: 10.1371/journal.pone.0056309

Figure 1. Definition of macaque NK cells and their response to Fc targets in the naive and SIV/SHIV infected macaques.

Figure 1

(A) Macaque NK cells were defined as CD3−CD8+NKG2A+. 3 subsets were further divided by the expression of CD16 and CD56. Levels of CD16 expression on NK cells (B) as well as CD107a (C), IFN-γ and TNF-α (D) were measured. The flow plots represent the capacities of macaque NK cells to respond to medium alone, the mouse cell line p815, p815 cells coated with p815-specific Abs (Fc targets) and PMA+Ionomycin (from left to right ) respectively. (E) Correlation between CD16 MFI after stimuli by Fc targets and functional molecules are illustrated. Spearman Rank Order Correlation coefficients ‘R’ and corresponding ‘P values’ are indicated. (F) Chronic SIV/SHIV infection was associated with compromised NK cell-mediated responses to Fc targets in terms of the intensity of CD16 after stimulation (left; Horizontal bars indicate means for the normality data analyzed by Student's t-test ;*P<0.05 ) and the production of functional molecules (right; Horizontal bars indicate medians for the non-normality data analyzed by Mann-Whitney U-test; ***P<.001).