Figure 6. Inactivation of Escherichia coli O157:H7 by combined processes of heat and Pulsed Electric Treatments with (+)-limonene.

Log₁₀ of survival counts of Escherichia coli O157:H7 after lethal heat treatments (54° C for 10 min) (A) or Pulsed Electric Fields treatments (30 kV/cm and 25 pulses) (B) in absence (□) or presence of 200 µL/L of (+)-limonene (▪) and recovery onto TSAYE. Cells after heat and PEF treatments without (+)-limonene were also recovered onto TSAYE-SC (sodium chloride: vertical stripes) or TSAYE-BS (bile salts: horizontal stripes). Discontinuous line indicates initial cell concentration (3·10⁷ CFU/mL). Arrows indicate survival counts under detection limit. Error bars indicate standard error.