Skip to main content
. 2004 Feb 5;101(7):2040–2045. doi: 10.1073/pnas.0307301101

Fig. 4.

Fig. 4.

Repression of the intensity of luciferase luminescence of ARE-luciferase in K0N0 mouse embryonic fibroblasts by wild-type Keap1 and its cysteine mutants. All cells were transfected with the ARE-luciferase (2 μg) and the Nrf2 (8 μg) constructs. (a) Repression of luminescence as a function of amount of wild-type Keap1 (black bars) and its reversal by exposure to 2 μM sulforaphane (gray bar). (b) Repression of luminescence by wild-type (WT) Keap1 (2 μg) and equivalent quantities of the following mutants: NTR (C23A and C38A); CTR (C613A, C622A, and C624A); IVR C226A–C297A (C226A, C241A, C249A, C257A, C273A, C288A, and C297A); IVR C226A–C249A (C226A, C241A, and C249A); and IVR C257A–C297A (C257A, C273A, C288A, and C297A). (c) Repressive activity of single or multiple cysteine mutants of Keap1 with 100 ng of each expression vector. The structures of the mutants are designated: + for cysteine, – for alanine. The structure of each wild-type and mutant Keap1 is shown below the bar indicating its repressor activity. Repressor activity is abrogated if C273 or C288, or both, are mutated to alanine.