Figure 5. As3+ induces cytosolic localization of S21-phosphorylated EZH2. (A) Immunofluorescent staining of EZH2 in control cells or cells treated with 20 μM As3+ for 2 h. (B) Immunofluorescent staining of S21-phosphorylated EZH2 (pEZH2) in cells cultured in the presence or absence of 20 μM As3+ for 2 h. Note the punctuation pattern of pEZH2 in the cytoplasm. (C) Nuclear or cytosolic localization of EZH2 and S21-phosphorylated EZH2 as determined by cellular fractionation. The purity of cytosolic fraction and nuclear fraction was validated by detecting GAPDH and Histone H3, respectively. The arrows indicate pEZH2; the blank triangle on the light of the top panel indicates non-specific bands in the nuclear fractions. Data are representative of three experiments.