Figure 1. Expression and function of GR in leukemia and lymphoma cell lines. (A) Expression of GR in leukemia and lymphoma cells was determined in untreated cells by western blot analysis of whole-cell lysates. Membranes were probed with anti-actin antibodies to verify equal protein loading and transfer. Image digitizing and quantitative analysis of GR: actin normalized expression was performed by the Odyssey v 1.2 software. (B) Cells were treated with solvent (Control) or Dex for 24 h, and GR nuclear translocation was determined by western blot analysis of nuclear proteins. Membranes were probed with anti-HDAC-1 antibodies to verify equal protein loading and transfer. (C) Cells were treated with solvent (Control) or Dex for 48 h, and effect on cell growth was estimated by cell counting. * Statistically significant differences (p < 0.05) between Dex- and solvent-treated cells. (D) Generation of cells with GR knockout. Cells were infected with shGR- or empty vector-expressing lentiviruses. Expression of GR in CEM-shEV, CEM-shGR, NCEB-shEV and NCEB-shGR cells was determined by western blot analysis of whole-cell lysates. Membranes were probed with anti-actin antibodies to verify equal protein loading.