Figure 5. Bortezomib induced GR accumulation, and enhanced CpdA properties as “dissociated” GR ligand. CEM and NCEB cells stably infected with lentiviruses expressing luciferase reporters: GRE.Luc (A), NFkB.Luc or AP1.Luc cells (B) were incubated for 8 h with solvent (control), Bortezomib (BZ), CpdA or BZ + CpdA. Luciferase activity was determined as described in “Materials and Methods.” Statistically significant difference (*p < 0.05; ** p < 0.01) between treated- and control cells; # statistically significant difference (p < 0.05) between CpdA and BZ+CpdA. (C). Cells were incubated for 8 h with BZ or solvent (Control), and GR expression was determined by western blot analysis of whole CEM and NCEB cell extracts. To verify equal loading and adequate transfer, the membranes were probed with anti-actin antibodies.