Figure 1. Phenotypic characterization of GBM TICs and differentiated cells. Upper pictures: Representative morphological appearance of GBM TICs grown as spheres, monolayers on matrigel or after FBS-dependent differentiation (enlargements 10X). Lower pictures: Expression of TIC (CD133, nestin) or differentiated cell (GFAP, Map2) markers. For spheres, sequential confocal microscopy z-plane sections are reported. The following picture enlargements were used: TIC spheres, 40X; TIC monolayers, 10X; differentiated GBM cells, 10X for top pictures and 100X for middle and lower pictures.