Figure 3. Silencing of Aurora A induces G₂/M arrest. (A) The growth of vector and Aurora A shRNA HepG2 cells were measured on the indicated days. Data shown are the mean ± SD of three independent experiments. (B) Cell cycle profile of synchronized vector control and Aurora A shRNA HepG1, Huh-7 and Hep3B cells was analyzed by flow cytometry analysis. Percent of G₂/M cells is shown. (C) The level of FoxO1, cyclin B1 and Aurora A during M phase was measured by western blot analysis. Nocodazole (100 ng/ml) arrested control and Aurora A shRNA HepG2 and Hep3B cells were released from nocodazole block, and protein lysates were prepared at the indicated time thereafter. Equal loading of protein was confirmed by GAPDH.