Figure 4. Depletion of FoxO1 in Aurora A-knockdown cells lead to exit G₂/M arrest and induce apoptosis. (A) FoxO1-specific siRNA was transfected into vector control and Aurora A shRNA HepG2 cells, and knockdown of FoxO1 was confirmed by western blot analysis. (B) Cell cycle profiles in scrambled or FoxO1-specific siRNA-transfected control and Aurora A-knockdown HepG2 cells were monitored at 72 h after transfection by flow cytometry analysis. Representative profiles and percent of subG₁, G₀/G₁, S, G₂/M cells from one of two independent experiments are shown. (C) The percentages of TUNEL-positive nuclei in scrambled or FoxO1-specific siRNA transfected control and Aurora A-knockdown cells 96 h after transfection. Data represent the means ± SD of two independent experiments.