Fig. 1.

Expression of WNK4 in extrarenal tissues. (a) RT-PCR analysis of WNK4. cDNA from indicated mouse tissues was used as a template for PCR by using primers specific for WNK4 (Upper) and GAPDH (Lower) mRNA, and the products were fractionated on agarose gels. (b) Western blot analysis of WNK4. Mouse or human tissue lysates were fractionated by SDS/PAGE and subjected to Western blot analysis with anti-WNK4. A protein of ≈135 kDa, the expected size of WNK4 (2), is not exclusively expressed in kidney, but is also present in brain, testis, colon, heart, liver, prostate, and lung. All lysates are from mouse except breast, ovary, colon, melanocytes (melano.), and prostate.